Device

Part:BBa_I742128:Design

Designed by: Caroline Dahl   Group: iGEM07_Edinburgh   (2007-10-22)


Cell replication --> YFP on/off


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Rumour (unpublished data) has it that the flanked DNA needs be of certain lenght to allow it to form a loop and subsequently invert. 200bp is minimal so the Plac was extended to include nonfunctional LacZ C-terminus.


Source

Dif-sites and the Plac are innate E. coli K12 loci. See BBa_E0430 for details about YFP.

References